EFFECT OF HCG ON HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMC) SECRETION OF PRO- AND ANTI-INFLAMMATORY CYTOKINES

Successful embryo implantation and appropriate trophoblast invasion is associated with an adequate communication interaction between peripheral blood mononuclear cells (PBMC) the human embryo. Human chorionic gonadotropin (hCG) one of earliest embryonic signals that affect PBMC cytokine secretion. Nowadays, intrauterine administration hCG-activated autologous a widely used treatment for improvement pregnancy outcome. However, effect hCG on secretion scarcely studied. The aim present study was to estimate activation pro- anti-inflammatory cytokines (TNF-a, IL6, IL4, IL10). Blood samples (8 ml) were collected from 36 women during mid-luteal phase cycle. isolated cultured (2x106 cells/well) in 0.5 ml RPMI-1640 medium (R8758, Sigma-Aldrich, Germany) + 1% serum albumin (HSA) without 10IU/ml (Choriomon, IBSA, Switzerland) 24h. Tumor necrosis factor alpha (TNFα), Interleukin-6 (IL-6), Interleukin-4 (IL-4) Interleukin-10 (IL-10) cell culture media determined by sandwich enzyme-linked immunosorbent assay (ELISA) (CSB-E04638h, CSB-E04740h, CSB-E04633h CSB-E04593h, Cusabio Technology, Wuhan, China, respectively). Statistical analysis conducted using SPSS v.21. A paired t-test comparison or hCG. p value less than 0.05 considered indicate statistically significant result. activated had no difference TNFa, IL-6 IL-4 after 24 hours compared In 53% (19/36) induced TNFα 61% (22/36) all cases rest presence resulted reduced contrast, increase IL-10 PBMCs (6154±781 pg/ml vs. 3437±446 pg/ml, P=0.04, Moreover, have led 78% (28/36) studied (Mean fold change 1.70). has shown respond secrete significantly higher quantities anti-inflamatory could potentially support invasion. divergent effects other pro-inflammatory (IL-6 TNFa) (IL-4). conclusion, patient-specific their profile.